TABLE 3.

MICs for recombinant full-length GIM-1 and mutants cloned in pCRBluntII-TOPO in E. coli TOP10 cells

Isolate	MICa (mg/liter)
	Penicillins			Cephalosporins					Monobactam	Carbapenems
	AMP	PIP	PTc	CAZ	CTX	CXM	FOX	FEP	ATM	MEM	IPM	ETP
TOP10	4	4	8	0.5	0.125	8	4	0.0625	0.125	0.0625	1	0.008
GIM-1	≥256	64	≥256	16	32	≥256	≥256	0.25	0.125	0.25	2	0.25
W228R	≥256	16	32	4	8	≥256	8	0.125	0.125	0.25	2	0.5
W228A	≥256	32	64	4	16	≥256	8	0.125	0.125	0.5	1	0.5
W228Y	≥256	≥256	64	8	16	≥256	64	0.25	0.125	0.5	1	0.5
W228S	≥256	32	64	8	16	≥256	8	0.0625	0.125	0.25	1	0.25
Y233N	≥256	128	≥256	16	32	≥256	≥256	0.5	0.125	0.125	4	0.125
Y233A	≥256	≥256	≥256	16	16	≥256	16	0.25	0.125	0.125	2	0.25
Y233S	≥256	64	≥256	16	16	≥256	8	0.25	0.125	0.25	1	0.25
Y233I	≥256	32	128	16	8	≥256	8	0.125	0.125	0.0625	1	0.0625

^aAMP, ampicillin; PIP, piperacillin; PTc, piperacillin-tazobactam; CAZ, ceftazidime; CTX, cefotaxime; CXM, cefuroxime; FOX, cefoxitin; FEP, cefepime; ATM; aztreonam; MEM, meropenem; IPM, imipenem; ETP, ertapenem.