TABLE 1.
Purpose | Gene target | Primer sequences (5′–3′) |
---|---|---|
Amplification | sdhB | GGTTCAAGCGCTCTCATGC and GTGGTAGCCAAGGGTATTTGC |
sdhC | CAATTCGATCAGACGCGAAG and GTCCATACTTCGATAAGGAAC | |
sdhD | GCTTCCCGAGGATGTTTTTCG and GGGTACAAAAAAGACAGAAATACAGG | |
Sequencing | sdhB | GAACATTGACGGAGTAAACAC, GCTGTACGAATGTATTCTCTG, and GTGTTTACTCCGTCAATGTTC |
sdhC | TATACCGACCGCAGATAACC and CTGGCGAAGATATAGAGACC | |
sdhD | AACCACTAACGATGCTGCTCC and GCAACGACTCTCTCAAAGG |