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. 2016 Jan 29;60(2):873–880. doi: 10.1128/AAC.01790-15

TABLE 1.

PCR primers used for genetic analysis of laboratory-generated ME1111-resistant mutants

Purpose Gene target Primer sequences (5′–3′)
Amplification sdhB GGTTCAAGCGCTCTCATGC and GTGGTAGCCAAGGGTATTTGC
sdhC CAATTCGATCAGACGCGAAG and GTCCATACTTCGATAAGGAAC
sdhD GCTTCCCGAGGATGTTTTTCG and GGGTACAAAAAAGACAGAAATACAGG
Sequencing sdhB GAACATTGACGGAGTAAACAC, GCTGTACGAATGTATTCTCTG, and GTGTTTACTCCGTCAATGTTC
sdhC TATACCGACCGCAGATAACC and CTGGCGAAGATATAGAGACC
sdhD AACCACTAACGATGCTGCTCC and GCAACGACTCTCTCAAAGG