FIG 3.

Effect of polyP3 on viability of preformed P. intermedia biofilm. Preestablished biofilms of P. intermedia ATCC 49046 were treated with polyP3 at the indicated concentrations for 24 h. (A) Biofilm biomass was quantitated by crystal violet staining. The viable biofilm bacteria were enumerated by detachment of cells, followed by agar plating count. The results are expressed as the means ± SDs from two independent experiments performed in triplicate. *, P < 0.05, versus value for control. (B) CLSM images of the P. intermedia biofilms. Magnification is ×600.