FIG 3.

Assay for proteolytic activity of promastigote protein lysates. (A and B) Protein lysates obtained from stationary-phase promastigotes were preincubated in the first incubation step (1st Inc.) with DMSO, 200 μM E-64, 200 μM CA074, 200 μM CLIK-148, 200 μM compound 13b, 200 μM compound 13e, or 200 μM compound s9. In the second incubation step (2nd Inc.), protein lysates were incubated with either DMSO, 200 μM compound 13b, 200 μM compound 13e, or 200 μM compound s9. Proteinase activities were determined by proteolytic degradation of the fluoropeptide Cbz-Phe-Arg-AMC.