TABLE 3.
Results of mutations in the pncA gene (Rv2043c) observed in PZA-resistant isolates
| Type of mutation | Mutation(s) in mouse strain: |
|
|---|---|---|
| BALB/c | C3HeB/FeJ | |
| Point mutations | K96 M | M1I, T135P |
| G132A | L19R, H137D | |
| C138Y | A46E, V139 M | |
| T142P | S67P, A146V | |
| L159V | H71Y, S164P | |
| H171R | C72F, L172P | |
| Y99stop, E173stop | ||
| G108R | ||
| I133N | ||
| Small indelsa | +A in E127 | −G in P54 |
| −CG in A26 | ||
| −T in I133 | ||
| −CGTCAGCGGTACTC in V73–P77 | ||
| Large-scale deletions | Rv2023c–Rv2048c (27 kb) | Rv2030c–Rv2048c (21 kb) |
| Rv2027c–Rv2047c (19.8 kb) | Rv2034–Rv2045c (9 kb) | |
| Rv2039c–Rv2048c (13 kb) | ||
| Rv2040c–Rv2045c (3.5 kb) | ||
| Rv2041c–Rv2043c (1.1 kb) | ||
| Rv2042c–Rv2043c (1 kb) | ||
| Rv2043c–Rv2044c (0.2 kb) | ||
| 5′ UTRb | A→G at −11 bp (upstream) | |
| No pncA mutation | One (A3311T mutation in Rv3350c) | |
a
Frameshifts.
b
UTR, untranslated region.