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(A) Burst in ceftazidime (25 μM) hydrolysis observed with 10 μM the C69F variant. Extrapolation (green line) of the steady-state velocity to the y axis revealed a burst amplitude of ∼1.5 μM. (B) Burst in ceftazidime (25 μM) hydrolysis observed with 10 μM the C69F variant that was preincubated with anti-PenI polyclonal antibody for 10 min. Extrapolation (green line) of the steady-state velocity to the y axis revealed a burst amplitude of ∼17 μM.
