Figure 2.

OOS reduces in vitro viability of C26 cells. (A) The viability of C26 cells was tested after 24-h incubation in the presence of OOS. C26 cells were treated with increasing concentrations of OOS ranging from 1:200 to 1:50 during 24 h before viability quantification and compared to the viability of initially cultures cells (light grey) and to that of untreated cells (black). Differences in the viability of treated cells vs. Untreated cells (*) and vs. initially cultured cells (**) were considered to be statistically significant at P<0.05. (B) The cell number in each cell phase respect to total gated cell number was analyzed after PI staining by flow cytometry after 72-h incubation with 1:100 OOS (V/Vf). Differences between treated and untreated cells were considered statistically significant at ^*P<0.05.