Fig. 2.

Mesoglycan-induced expressions of p53 and p21 inhibits cell cycle progression. Cells were treated with mesoglycan (0.1 μg/ml) or mesoglycan plus PDGF (10 ng/ml), and treated with PDGF for 24 h. Cell cycle analysis was assessed by PI staining and the percentage of cells in G₀/G₁ phase, S phase. Cells were detached by trypsinization and studied with flow cytometry (a). Cells were treated with mesoglycan for the indicated periods of time. Protein expressions of p53, p21, p27 and PCNA were determined by western blot analysis (b, c). *p <0.05 versus the control, ^† p <0.05 versus PDGF. Representative results from three independent experiments are shown