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. 2016 Feb 15;27(4):640–653. doi: 10.1091/mbc.E15-03-0137

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© 2016 Aillaud et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — The novel β2A/B-tubulin variant βΔ4 evidenced in HeLa cells by 3EG antibody. (A) Immunocytochemistry of HeLa cells using 3EG and anti–β-tubulin antibody (βtot). Scale bar: 10 µm. (B) Immunoblot of protein extracts from nonsynchronized HeLa cells and from HeLa cells synchronized in G1/S or mitosis (see Materials and Methods). Cell synchronization was verified using a phosphohistone antibody (H3-Ser10P). Loaded proteins were standardized using αtot antibody, and the truncated β-tubulin form was analyzed with 3EG antibody. (C) Analysis of the β-tubulin band in protein extracts from HEK293T cells expressing GFP- or YFP-tagged CCPs (complementary analysis of the experiment in Figure 3A, right, including lower band).