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. 2016 Feb 15;27(4):702–715. doi: 10.1091/mbc.E15-04-0257

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© 2016 Fan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — HGF-induced Akt/Erk phosphorylation is attenuated by NHE5 knockdown. Cells serum-starved overnight were stimulated with 50 ng/ml of HGF. The amount of phosphorylated and total Akt and Erk1/2 was detected by immunoblotting. (A) Representative immunoblots are shown. (B) The relative level of phospho-Akt and phospho-Erk are expressed as mean ± SEM of four experiments. p Values for pairwise comparisons at each time point were obtained following repeated-measures ANOVA with time as the within-subject factor. *, p < 0.05, **, p < 0.01, ***, p < 0.001, n.s., not significant.