Table 2. Influenza A RNA copies/m3 of air concentrations detected in the swine facilities using two personal bioaerosol samplers.
Personal samples were collected among veterinarians working in swine production facilities that were infected with influenza A (H1N1 or H3N2) virus. Swine veterinarians were called to the swine facilities during a suspected influenza A infection and collected bodily fluids for a diagnosis. Samples were collected at various time during or after this initial evaluation of the infected swine herd. The NIOSH bioaerosol sampler and the PHISH collected samples at a flow rate of 3.5 L/min and 10 L/min, respectively. Total RNA copies/m3 were determined by qPCR. The summary of the data is reported as geometric mean (GM) and geometric standard deviation (GSD).
Farm | Time elapsed after initial evaluation | NIOSH 15 mL(> 4μm) (RNA copies/m3) | NIOSH 1.5 mL (1–4μm) (RNA copies/m3) | NIOSH filter (<1μm) (RNA copies/m3) | NIOSH total (RNA copies/m3) | PHISH filter (RNA copies/m3) |
---|---|---|---|---|---|---|
1 | 0 days | 5471 | 767 | 70 | 6309 | 2481 |
2 | 2 days | 3491 | 1478 | 171 | 5140 | - |
3 | 7 days | 3708 | 1193 | Not detected | 4901 | 552 |
4 | 14 days | 390 | 35 | Not detected | 425 | 119 |
5* | 14 days | 582 | 14 | Not detected | 596 | Not detected |
GM(GSD) | 1742 (3) | 232 (9) | 110 (2) | 2094 (4) | 545 (5) |
* RT-qPCR Ct values from all Farm 5 samples were outside the linear range of the calibration curve