Fig 1. A smaller molecular mass form of BBS5 is detected in retinal extracts from a variety of vertebrates.

(A) Immunoblots of extracts of aqueous soluble proteins prepared from whole retinas from the indicated species probed with anti-BBS5 #7–15 monoclonal antibody described in [19]. Arrow indicates the expected molecular size of BBS5; arrowhead indicates a smaller immunoreactive band at 26 kD. (B) A replicate blot was probed with the same antibody that was pre-incubated with heterologously-expressed recombinant murine BBS5. Inset, at the bottom of the blot shows a replicate blot stained for beta-tubulin as a loading control (beta-tubulin indicated with an open arrow).