Fig 4. RT-PCR of predicted Bbs5 transcripts from retinal cDNA.

The alternative transcripts for mouse Bbs5 predicted in the Ensembl (Bbs5-002 through -008) and MGI (Bbs5-X1) were amplified with primers that would selectively identify the alternative transcript. The predicted sizes (in bp) for each transcript are indicated below each lane for the full-length transcript and the alternative splice variant transcript [two alternative transcripts are possible in lane 1, corresponding to the deletion of Exon 8 (152 bp) or the inclusion of Exon 7A (379 bp)]. Lower panel shows amplification reactions with primers against alpha-tubulin to control for cDNA quality.