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. 2016 Feb 11;11(2):e0148773. doi: 10.1371/journal.pone.0148773

Fig 6. BBS5L localizes to the axonemal structure of the photoreceptors.

Fig 6

(A) Lower magnification view of indirect immunofluorescence of antibodies against BBS5L (green), BBS5 (red), RP1 (white), and DAPI (blue) on cryofixed mouse retinal tissue (see methods for specific details on antibodies). (B-E) Enlargement of region indicated in (A) showing localization of BBS5L (B) and BBS5 (C). BBS5L and BBS5 reactivity are highly overlapping as seen in the merge (D). Staining with the axonemal marker RP1 (E) shows that BBS5L co-localizes with RP1 in the distal axoneme (F), but also stains the proximal transition zone of the axoneme where RP1 is absent (white arrowheads). G-L shows parallel staining with pre-absorption controls, using anti-BBS5L antiserum preabsorbed against BBS5L protein (H) or anti-BBS5 antibody preabsorbed against BBS5 protein (I). OS, outer segments, IS, inner segments, ONL, outer nuclear layer; scale bars are 10 μm (A) and 2.5 μm (B-F).