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. 2015 Nov 28;149(2):503–515. doi: 10.1093/toxsci/kfv254

FIG. 1.

FIG. 1.

Intoxication of diverse neuron cultures with BoNT/A or BoNT/B blocks spontaneous neurotransmission and results in SNARE protein cleavage. (A) Representative whole-cell recordings, cumulative probability distributions of inter-event intervals and mean mEPSC frequencies demonstrating spontaneous synaptic activity in ESNs, hSNs, CtNs, and CNs (n = 14–30 for each). Scale bar = 2 s × 60 pA for ESNs and 20 s × 60 pA for others. (B) ICC confirming expression and localization of neurotypic markers NeuN (orange and MAP2 (magenta) as well as CNT target proteins SNAP-25 (green) and Syb2 (white). Scale = 20 µm (C) Representative mEPSC recordings and (D) normalized mEPSC frequencies demonstrating reduced spontaneous release in cultures treated with BoNT/A or BoNT/B but not formalin-inactivated toxoid (n = 8–16 each). Scale is same as panel A. Data are presented as mean ± SEM. *P < 0.05. ***P < 0.001. (E) Immunoblot demonstrating changes in SNAP-25, Syb2 and Stx1 following treatment with BoNT/A or BoNT/B but not formalin-inactivated toxoid.