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. 2015 Nov 28;149(2):503–515. doi: 10.1093/toxsci/kfv254

FIG. 2.

FIG. 2.

BoNT/A-G or TeNT intoxication blocks synaptic neurotransmission in ESNs. Treatment with BoNT/A-G or TeNT reduces or eliminates spontaneous release after 20 h, demonstrated in (A) representative whole-cell recordings and (B) mean normalized mEPSC frequencies. Scale bar = 2.5 s × 60 pA (n = 8–16 each). ***P < 0.001. (C) Representative immunoblot demonstrating cleavage of SNAP-25 (S25) and loss of immunoreactivity of Stx1 in cultures treated with BoNT/A, /C or /E (n ≥ 4). (D) Representative immunoblot demonstrating loss of Syb2 immunoreactivity following treatment with BoNT/B, /D, /F, /G, and TeNT (n ≥ 4).