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Use of synaptic neurotransmission to determine toxin serotypes and evaluate antitoxin specificity. Pre-incubation of toxin with serotype-specific antitoxin protects spontaneous release frequencies and SNARE protein integrity at 20 h after intoxication. A, B, Representative whole-cell recordings. C, D, Normalized mEPSC frequencies. E, F, Representative immunoblots. Scale bar = 2.5 s × 60 pA. Normalized data are from 12 to 20 recordings and presented as mean ± SEM. ***P < 0.001.
