Figure 2.

Immunocytochemistry for cell-type specific markers. Horizontal sections through the vallate papilla. (A–D) Triple label for Villin (red; A and C) and the Type II markers GNAT3 (gustducin; blue; A and B), and PLCβ2 (green; A and D). (E–J) Longitudinal sections of vallate papillae where dorsal is to the left. (E–G) Double label for SNAP25 (red; E and F) and Car4 (green; E and G). SNAP25 labels nerve fibers well but does not obviously label any taste cells. Car4 labels only rare taste cells (arrow G). (H–J) T1R3 (Tas1R3) labels several elongate taste cells in nearly every taste bud. Note the presence of taste buds on both sides of the crypt in J.