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cPLA2α gene disruption protects against angiotensin II (Ang II)-induced increase in renal oxidative stress. NADPH oxidase activity measured in kidney homogenate (A), urinary thiobarbituric acid reactive substances (TBARS) (B), ROS production determined by DHE staining (C) quantified as fluorescence of 2-OHE (D). *P < 0.05 cPLA2α+/+ vehicle vs. cPLA2α+/+ Ang II, # P < 0.05 cPLA2α+/+ Ang II vs. cPLA2α−/− Ang II (n = 6 for each group, data are expressed as mean ± SEM).
