Table 1.

Pseudoalteromonas environmental isolates, their origins and their resemblance to type strains of known species according to 16S rRNA gene sequence and MALDI-TOF mass spectrum.

Isolate	Origin	16S rDNA Accession	EzTaxon		Biotyper
			Closest species	Differences/Total length	Closest species	Score
DG1135	Gymnodinium catenatum (dinoflagellate) algal culture	AY258115	P. phenolicaa	10/1382	P. translucida	2.19
DG1152	Pseudo-nitzschia multiseries (diatom) algal culture	AY548764	P. marina	2/1371	P. marina	2.02
DG1425	Pseudo-nitzschia seriata (diatom) algal culture	KF482376	P. marina	2/1319	P. carrageenovora	2.01
DG1628	Scottish seawater oil-enrichment	EU239912	P. carrageenovora	0/1383	P. carrageenovora	2.41
DG1664	Scottish seawater oil-enrichment	EU239890	P. elyakovii	0/1385	P. elyakovii	1.97
DG1810	Celtic Sea oil-enrichment	KF482377	P. tetraodonis	0/1347	P. haloplanktis	2.31
DG1838	Celtic Sea oil-enrichment	KF482378	P. elyakovii	6/1377	P. haloplanktis	2.19
TG12	Scottish seawater oil-enrichment	EF685033	P. arctica or P. translucida	9/1438 or 9/1437	P. elyakovii	2.00
NS-07	North Sea	AB607136	P. prydzensis	14/1359	P. mariniglutinosa	1.88
NS-10	North Sea	AB607137	P. tetraodonis or P. issachenkonii	2/1396 or 2/1395	P. haloplanktis	2.25
NS-20	North Sea	AB607145	P. undina	2/1377	P. haloplanktis	2.27
NS-34	North Sea	AB607157	P. undina	4/1412	P. haloplanktis	2.38
NS-36	North Sea	AB607158	P. prydzensis	19/1449	P. prydzensis	2.08

The EZTaxon database was queried with the sequences identified by their accession numbers. Biotyper scores are for comparisons to the MALDI-TOF MS library developed in house. Scores ≥2.30, indicating “confident” species identification according to Bruker Daltonics (MALDI Biotyper 3.1 User Manual, Bruker Daltonics, 2012), are given in bold.

^aNot included in this study.