Figure 3.

Bacterial burden in lungs. Groups of female Balb/c mice (n = 6) were immunized subcutaneously with 20 μg FilF formulated with CFA/IFA adjuvant on day 1, 14, and 21. The mice were intra-tracheally challenged with 10⁸ CFU of A. baumannii ATCC 19606 at day 29. Immunization with FilF reduced the bacterial burden by 2 and 4 log cycles in the lungs of pneumonia model mice sacrificed 12 and 24 h post infection, respectively. The data are presented as mean ± SD (n = 6). p-value was determined by the one way analysis of variance (ANOVA). ^***p < 0.001 (Adjuvant control vs. FilF immunized mice).