Figure 5.

Cytokine levels in the sera. Groups of female Balb/c mice (n = 6) were immunized subcutaneously with 20 μg FilF formulated with CFA/IFA adjuvant on day 1, 14, and 21. The mice were intra-tracheally challenged with 10⁸ CFU A. baumannii ATCC 19606 at day 29, and sacrificed at 12 and 24 h post-challenge. The detection limit for all cytokines and chemokines is <10 pg/ml. p-value was determined by the one way analysis of variance (ANOVA). ^*p < 0.1, ^**p < 0.05, ^***p < 0.001, ^#non-significant, (Adjuvant control vs. FilF immunized mice).