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Antibiofilm activity of cell free supernatants from marine actinobacteria. The pre-formed biofilm on glass slides was treated for 24 h with 150 μg each of freeze-dried cell free supernatant (CFS). Untreated biofilm (control) and remaining biofilm of V. harveyi treated after CFS on glass slides were stained with 0.1% acridine orange and observed in CLSM (LSM 710, Carl Zeiss).
