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All Tspan14-CD9 chimeras partially co-localize with ADAM10 and so have access to the metalloprotease. HeLa cells were transfected with the indicated Tspan14-CD9 chimeras (see Fig. 2A) and HA-tagged mouse ADAM10. Cells were fixed and stained with an anti-HA antibody (green) and an anti-FLAG antibody (red). Confocal microscopy images are representative of three independent experiments and at least 15 fields of view.
