FIGURE 1.

The insect peptide CopA3 enhances the proliferation of HT29 cells. A, HT29 cells (10⁵ cells/well) were treated with different concentrations of CopA3 for 48 h, and cell proliferation was measured by BrdU cell proliferation assays. The results represent the means ± S.E. of three experiments performed in triplicate. *, p < 0.01. B, HT29 cells were treated with medium (con), EGF (100 ng/ml) or CopA3 (20 μg/ml) for 48 and 72 h, and cell proliferation was assessed by BrdU cell proliferation assays. *, p < 0.005; #, p < 0.001. C, HT29 cells were treated with medium, EGF, or CopA3 for 48 h, and DNA synthesis was measured by PI staining and FACS. D, HT29 cells were treated with P4 (20 μg/ml) for 48 h. E, HT29 cells were incubated with CopA3 (20 μg/ml) for 24 and 48 h, and cell viability was measured by MTT assay. The bars represent the means ± S.E. from three experiments performed in triplicate.