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Dimerized CopA3 is detected in the cytosol of human colonocytes. A, HT29 cells were treated with CopA3 (20 μg/ml) for 2 h and separated into cytosolic (cyt), nuclear (nuc), and membrane (mem) fractions, which were probed for purity using primary antibodies against Sp1, GAPDH, and EGF receptor (EGFR), respectively. The presented results are representative of three separate experiments. B, HPLC analysis revealing the cytosolic location of CopA3.
