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MNK binding to eIF4G per se does not affect eIF4E binding to eIF4G. Binding mixtures containing constant amounts of eIF4G(589–1600) and eIF4E(S209A) (lanes 1–4) or eIF4E(S209D) (lanes 5–8) were incubated with S-protein-agarose in the absence or presence of increasing amounts of MNK1 (A) or MNK2 (B). Bound proteins were purified and analyzed by Western blotting as described under “Experimental Procedures.” Results are representative of two independent experiments.
