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Expression of a β-galactosidase reporter under control of the TnrA-dependent nrgA promoter reveals transcriptional activity of TnrA. B. subtilis cells were grown under nitrogen-limited conditions using either 1.5 mm glutamine (circles) or 20 mm nitrate (squares) for strain GP250 (wild type background) and using 1.5 mm glutamine (triangles) for strain GP251 (glnA− background). Error bars, S.E.
