FIGURE 6.

SPR analysis of TnrA binding with 30-mer nrgAB or 54-mer glnRA promoter fragment. A, TnrA was injected onto the immobilized DNA surface (thin continuous line for the nrgAB promoter, thin dashed line for the glnRA promoter) alone or in a mixture with GS in a 3:1 ratio (continuous line for the nrgAB promoter, dashed line for the glnRA promoter). B, TnrA was injected onto an immobilized nrgAB promoter fragment (continuous line) or glnRA promoter fragment (dashed line), and then GS was loaded onto the TnrA-DNA chip surface. C, TnrA was injected onto an immobilized nrgAB promoter fragment; subsequently, GS was injected onto the immobilized TnrA-DNA surface without effector molecules (continuous line), in the presence of 1 mm glutamine (dashed line) or 1 mm ATP (dotted line) and in the presence of 1 mm ATP and 1 mm glutamine (thin dashed line). D, TnrA was injected onto immobilized nrgAB promoter fragment; subsequently, GS was injected without effector molecules (continuous line), in the presence of 1 mm MSX (dashed line) or 1 mm ATP (dotted line) and in the presence of 1 mm ATP and 1 mm glutamine.