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. 2015 Dec 3;291(7):3483–3495. doi: 10.1074/jbc.M115.680991

FIGURE 7.

FIGURE 7.

SPR analysis of GS binding to TnrA immobilized on the 30-mer nrgAB promoter fragment in the presence of different effector molecules. A, GS was loaded onto the TnrA-DNA chip surface without effector molecules or in the presence of 1 mm MSX, 1 mm ATP, 1 mm ATP plus 1 mm MSX, 1 mm ADP, 1 mm ADP plus 1 mm MSX, 1 mm AMP, and 1 mm AMP plus 1 mm MSX, as indicated. B, GS was loaded onto the TnrA-DNA chip surface without effector molecules or in the presence of 1 mm MSX, 1 mm AMP-PNP, 1 mm AMP-PNP plus 1 mm MSX, 1 mm ATPγS (dotted line), or 1 mm ATPγS plus 1 mm MSX, as indicated.