FIGURE 8.

Influence of glutamine or AMP on GS interaction with TnrA immobilized on the 30-mer nrgAB promoter fragment. GS in the presence or absence of various effector molecules was injected onto surface-immobilized TnrA-DNA complex. The increase in RU relative to a control injection with GS in the absence of the variable effector molecule was recorded (normalized ΔRU). Glutamine was used as a variable effector molecule in A, C, and E, and AMP was used as a variable effector molecule in B, D, and F. No additional effector molecules were present in A and B. ATP at a constant concentration of 1 mm was present in C and D, and a constant concentration of 1 mm ATP and 10 mm glutamate was present in E and F. The obtained values were fitted to the equation for one-site-specific binding with Hill slope using GraphPad Prism.