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. 2015 Dec 3;291(7):3483–3495. doi: 10.1074/jbc.M115.680991

FIGURE 9.

FIGURE 9.

Size exclusion chromatography of cross-linked GS, TnrA, and GS-TnrA complexes. A, the elution profiles of GS (Ve = 1.14, 1.45, and 1.59 ml), TnrA (Ve = 1.66 ml), a mixture containing GS and TnrA (1 dodecamer:6 dimers) in the presence of 1 mm MSX (Ve = 1.12, 1.45, and 1.59 ml) or in the presence of 1 mm MSX and 1 mm ATP (Ve = 1.14, 1.45, and 1.59 ml) are shown. B, the elution profiles of GS (Ve = 1.11, 1.43, and 1.58 ml), TnrA (Ve = 1.64 ml), and a mixture of GS and TnrA (1 dodecamer:6 dimers) in the presence of 1 mm glutamine (Ve = 1.12, 1.45, and 1.59 ml). The elution peak at 0.9 ml most likely corresponds to protein aggregates. C, SEC-MALS profiles for GS and the GS-TnrA complex. Both preparations were separated by size exclusion chromatography, and the measured molar masses are shown as dotted (GS-TnrA complex) or dotted and dashed lines (GS) below the peak fractions.