FIGURE 6.

Comparison of the putative specificity channel of TE2 to that of TE1. A, surface representation of TE2 and the underlying residues that contribute to the putative specificity channel. The capping domain occludes access to the catalytic triad and surface channels. B, the Orlistat-TE1 complex and the residues that contribute to the specificity channel. In both cases the majority of the residues that interact with the acyl chain are located in the capping domain, a unique surface-loop insert for both enzymes. Orlistat also interacts with the TE1 surface through the interface cavity and short chain pocket, as described by Pemble et al. (PDB code 2PX6) (18). The putative pantetheine channel and ACP-interaction surface are also indicated.