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. 2015 Dec 14;291(7):3541–3551. doi: 10.1074/jbc.M115.675900

FIGURE 2.

FIGURE 2.

NGF protein secretion by NP and AF after cytokine and TLR agonist-treatment. Conditioned cell culture media were collected 12, 24, and 48 h after treatment with IL-1β, TNF, PGN, and LPS. NGF protein levels in the media was analyzed by ELISA (A and B) and Western blot (C–F). NP (A, C, and E) and AF (B, D, and F) media were both analyzed. Conditioned media after 48 h of treatment were used for Western blots. Recombinant human mature NGF (rhmNGF) and proNGF (rhproNGF) were used as controls for Western blots. Western blots were analyzed by densitometry and normalized to untreated cells in E (NP cells) and F (AF cells). Data were analyzed using the Kruskal-Wallis test for multiple comparisons of non-parametric data. Data are presented at the mean ± S.E. n = 4 for ELISA. n = 3 for Western blot. *, p < 0.05; **, p < 0.01; ***, p < 0.001.