FIGURE 7.

NGF secretion after p38, ERK1/2, and NF-κB inhibition. Small molecule signaling inhibitors SB203580 (10 μm, p38), PD98059 (10 μm, MEK), SB203580 and PD98059, or BMS-345541(5 μm, NF-κB) were added to cultures 2 h before treatment with IL-1β or PGN. Protein was precipitated from conditioned culture media after 48 h of treatment. NGF secretion after p38 or NF-κB inhibition in NP and AF cells (A) and after ERK1/2 or ERK1/2 and p38 in NP cells (B) was assessed by Western blot. Western blots were analyzed using densitometry for both NP (C)- and AF (D)-conditioned media. Data were normalized to NGF secretion from cells treated with IL-1β or PGN but no signaling inhibitors. IKK, inhibitor of κ B kinase. *, p < 0.05.