TABLE 2.
Summary data of the surface expression of all Kv1.3 and Kv1.5 constructs
Data are the mean ± S.E. (n). Statistical differences with the corresponding wild type channel (Kv1.3 for the Kv1.3 constructs and Kv1.5 for Kv1.5 constructs) are indicated. For Kv1.3 constructs significant differences were obtained with one-way ANOVA followed by Tukey's HSD. For Kv1.5 channels, a nonparametric Kruskal-Wallis one-way ANOVA was used followed by a pairwise MWW test with Bonferroni correction. Significant differences are depicted in bold.
| Kv1.3 constructs | % Cell surface expression | Kv1.5 constructs | % Cell surface expression |
|---|---|---|---|
| Kv1.3 | 39.82 ± 2.28 (23) | Kv1.5 | 37.72 ± 1.97 (40) |
| Kv1.3- T439A | 43.31 ± 2.37 (11) | K5C3 | 96.11 ± 4.01a (10) |
| Kv1.3 Y447A | 36.80 ± 4.78 (13) | K5N3 | 21.11 ± 1.6b (25) |
| Kv1.3-S459A | 42.11 ± 4.81 (13) | K5N3 + Kvβ2 | 27.32 ± 2.83c (20) |
| Kv1.3-S470A | 47.39 ± 3.26 (12) | Kv1.5-YS532 | 44.75 ± 5.6 (18) |
| Kv1.3-S473A | 46.51 ± 3.25 (11) | Kv1.5-YS613 | 40.58 ± 4.4 (15) |
| Kv1.3-S475A | 42.95 ± 4.31 (12) | ||
| Kv1.3 Y477A | 34.14 ± 2.55 (11) | ||
| Kv1.3-T493A | 39.97 ± 1.76 (22) | ||
| Kv1.3-YS | 30.31 ± 3.3c (15) |
a p < 0.001.
b p < 0.01
c p < 0.05.