TABLE 1.
Summary of calculated sedimentation coefficients and Stokes radii
| Species | Predicted s20,wa | s20,wa | Stokes radius |
Calculated mass (protein)b | ||
|---|---|---|---|---|---|---|
| n-Octylglucoside | Dodecyl-β-d-maltoside | Triton X-100 | ||||
| S | S | nm | kDa | |||
| p250 | 11.4 | 6.15 ± 0.20 | 10.38 ± 0.34 | 9.82 ± 0.16 | 10.09 ± 0.07 | 241 ± 12 |
| p160 | 7.4 | 5.27 ± 0.46 | 8.76 ± 0.19 | 8.41 ± 0.08 | 8.84 ± 0.08 | 176 ± 18 |
| Mα+Mβ′ | 5.9 | 3.86 ± 0.33 | 8.63 ± 0.12 | 7.96 ± 0.12 | 8.12 ± 0.06 | 130 ± 11 |
| Mα | 5.4 | 3.49 ± 0.31 | 8.03 ± 0.29 | 7.54 ± 0.09 | 7.68 ± 0.08 | 107 ± 10 |
a Predicted sedimentation values based on comparison with the globular standard most similar in molecular weight using the formula S1/S2 = (Mr1/Mr2)2/3. Actual sedimentation values are based on the distance traveled relative to globular standards. Standards and values were: ovalbumin (3.6 S, Mr 42,881), rabbit IgG (7.1 S, Mr 150,000), bovine liver catalase (11.3 S, Mr 247,000), and/or bovine thyroglobulin (19.3 S, Mr 667,000).
b Calculated based on s20,w and Stokes radius in octylglucoside and assuming association of p250 and p160 with a single detergent micelle as described under “Experimental Procedures.“