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. 2015 Dec 18;291(7):3639–3647. doi: 10.1074/jbc.M115.679704

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FIGURE 3. — DMF inhibits the intrinsic NFκB activity in MS culture of breast cancer cells. A, p65 DNA binding activity was measured via ELISA in a conventional adherent 2D culture of MCF-7 cells or MS culture with or without inhibitors (IKK7 1 μm or DMF 50 μm) added for the last 3 h. B, expression of the TNF, CCL2, and ICAM1 genes after 6 h of drug treatment was measured in the same groups described in A. The different letters above the columns indicate significant difference between treatments (p < 0.001). C, DMF (20 μm) up-regulates the expression of HMOX1 mRNA in both 2D and MS of MCF-7 cells. Data are shown as -fold change compared with vehicle (Veh) control. D, compound 16 (Comp 16, 1 and 10 μm) up-regulates the expression of HMOX1 mRNA in MCF-7 cells. E, MS formation is measured in MCF-7 cells treated with compound 16. *, p < 0.01; ***, p < 0.001.