FIGURE 5.

The double bond reactivity of DMF is required to inhibit the NFκB pathway in breast cancer cells. A and B, NFκB-RE activity (A) and ICAM1 (B) gene expression were measure in MCF-7 cells upon treatment with DMF or DMS, 20 μm each, as described in Fig. 1. Data are plotted as percent of TNFα alone. The different letters above the columns indicate a significant difference between treatments (p < 0.001). C, NAC (dashed line) reverses the inhibitory effect of DMF on the TNFα-induced expression of the NFκB target genes TNF, CCL2, and ICAM1 in MCF-7 cells. NAC (500 μm) was added 30 min prior to DMF and TNFα treatment.