Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 9;291(7):3648–3657. doi: 10.1074/jbc.M115.673640

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Analysis of binding of recombinant Mxr1p^N150 and Mxr1p^N400 to pACS-MXREs by EMSA. A, nucleotide sequence of oligonucleotides containing wild-type and mutant MXREs. MXREs are boxed. B, schematic of histidine-tagged Mxr1p^N150 and GST-tagged Mxr1p^N400. C, SDS-PAGE profile of Mxr1p^N150 and Mxr1p^N400 purified from E. coli lysates by nickel-nitrilotriacetic acid and glutathione affinity chromatography, respectively. D, analysis of binding of Mxr1p^N150 and Mxr1p^N400 to ³²P-labeled oligonucleotides containing wild-type and mutant MXREs by EMSA. Complexes I and II are generated when Mxr1p^N150 and Mxr1p^N400 bind to both MXREs. Only complex I is obtained when Mxr1p^N150 and Mxr1p^N400 bind to only one of the MXREs. E, supershift of complexes I and II formed by His-tagged Mxr1p^N150 by the addition of anti-His antibodies. F, abrogation of the formation of complexes I and II by GST-tagged Mxr1p^N400 by addition of anti-GST antibodies.