FIGURE 2.

Phospholipid and cholesterol distribution in SF9 and HEK 293T membranes. A–E, lipids extracted from SF9 (gray) and HEK 293T (black) membranes were analyzed on a rapid resolution LC system coupled to an ESI-QTOF mass spectrometer. Species were identified by Mass Hunter Qualitative Analysis software of auto-MS/MS data with an accepted maximal error of 5 ppm for detected m/z and quantification made by automatic integration of peaks from extracted ion chromatograms. Bars represent values normalized to 100% (w/w). A, phospholipid distribution; B, cholesterol to phospholipid ratio; C, PE (including PEO-) to PC (including PCO-) ratio; D, fatty acid chain length distribution; E, unsaturation(s) per acyl chain in main fatty acid chains. Error bars indicate S.D. (n = 3). p values were estimated by Student's t test (n = 3): ***, p < 0.001; **, p < 0.005.