TABLE 2.
Fatty acid chain length distribution in single PL species of SF9 and HEK 293T membranes
Fatty acid chain length of lipids extracted from SF9 and HEK 293T membranes in single PL species PC, PE, PG, PS, PI, and SM was normalized to 100% (w/w) and represented by mean ± S.D. (n = 3). N.F., not found.
| SF9 | HEK | |
|---|---|---|
| PC | ||
| C14 | 5.3 ± 1.7 | 6.2 ± 1.6 |
| C16 | 44.1 ± 7.8 | 48.6 ± 4.3 |
| C18 | 48.9 ± 7.1 | 43.1 ± 0.1 |
| C20 | 0.6 ± 0.3 | 1.5 ± 2.1 |
| C22 | N.F. | 0.1 ± 0.1 |
| Others | 1.0 ± 1.3 | 0.5 ± 0.6 |
| PE | ||
| C14 | N.F. | N.F. |
| C16 | 41.0 ± 0.2 | 32.2 ± 2.8 |
| C18 | 45.9 ± 0.1 | 59.9 ± 4.7 |
| C20 | 11.0 ± 0.4 | 7.5 ± 7 |
| C22 | 2.0 ± 0.1 | 0.4 ± 0.3 |
| Others | N.F. | N.F. |
| PG | ||
| C14 | N.F. | N.F. |
| C16 | 80.2 ± 3.3 | 12.2 ± 4.4 |
| C18 | 19.8 ± 3.3 | 87.8 ± 4.4 |
| C20 | N.F. | N.F. |
| C22 | N.F. | N.F. |
| Others | N.F. | N.F. |
| PS | ||
| C14 | N.F. | N.F. |
| C16 | 29.4 ± 3.7 | 24.4 ± 4.8 |
| C18 | 33.4 ± 0.9 | 32.0 ± 3 |
| C20 | 10.7 ± 2.3 | 18.6 ± 2.7 |
| C22 | 20.0 ± 1.4 | 23.8 ± 0.8 |
| Others | 6.5 ± 0.8 | 1.2 ± 1.9 |
| PI | ||
| C14 | N.F. | N.F. |
| C16 | 44.8 ± 1.3 | 14.5 ± 4.3 |
| C18 | 53.5 ± 0.7 | 76.7 ± 3.4 |
| C20 | 1.6 ± 0.7 | 8.5 ± 0.9 |
| C22 | 0.1 ± 0.0 | 0.3 ± 0 |
| Others | N.F. | N.F. |
| SM | ||
| C14 | 2.3 ± 3.3 | 2.1 ± 2.4 |
| C16 | 27.5 ± 0.4 | 44.7 ± 3.4 |
| C18 | 68.0 ± 3.5 | 50.2 ± 2.7 |
| C20 | N.F. | N.F. |
| C22 | N.F. | N.F. |
| Others | 2.2 ± 0.0 | 3.0 ± 3.3 |