FIGURE 2.

Overall structure of Rv2837c. A, cartoon diagram depicting the binary Rv2837c, in which Rv2837c is shown in cyan. Secondary structure elements of Rv2837c mentioned in the text are labeled. Rv2837c consists of the N-terminal DHH domain and the C-terminal DHHA1 domain. A linker region connects two distinct domains. B, distribution of the B-factor of the Rv2837c-C structure. The B-factor is colored from high (red) to low (blue). The DHH and DHHA1 domains are indicated. C, the predicated structure of the Rv2837c homodimer by PISA. Each subunit is shown as a cartoon and in different colors. The metal ions are shown as magenta spheres. D, stereo view of Mn²⁺ cations at the active site of Rv2837c. The residues in contact with the metal ions are shown as sticks along the Rv2837c ribbon (cyan). In addition, Wat¹ is a bridging ligand to both Mn²⁺ ions. Wat² and Wat³ are present to complete the coordination state of two Mn²⁺ ions. Mn1²⁺ and Mn2²⁺ are denoted by magenta spheres, whereas water molecules are shown as red spheres.