FIGURE 9.

Rv2837c decreases both c-di-AMP and c-di-GMP levels in vivo. A, determination of intracellular c-di-AMP and c-di-GMP concentrations of ΔMSMEG_2630/pMV261 and OERv2837c strains by LC-MS/MS. 50 μg ml⁻¹ of kanamycin was added to stabilize the pMV261 plasmids. B, determination of intracellular c-di-GMP concentrations of E. coli MG1655/pMV261 and OERv2837c strains by LC-MS/MS. C, swimming phenotypic effects of OERv2837c. Swimming motility in LB after 10 h at 42 °C was measured; the diameter of the diffuse growth zone is indicated by the histogram.