Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 12;6:20923. doi: 10.1038/srep20923

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Cytotoxicity of free DFO (black square) and oxNG2-DFO (red circle) on normal J774A.1 cells (A) and in iron overloaded J774A.1 cells (B) after 48 h incubation; a representative set of data is shown for oxNG2-DFO where each data point is presented as the mean ± SD (n = 3). (C) Ferritin reduction assay to monitor iron chelation efficacy of DFO and oxNG2-DFO in iron overloaded J774A.1 cells. Iron overload was induced by 24 h incubation with 100 μM FAC. Cells were then treated with DFO or oxNG2-DFO at 10 μM or 50 μM for 48 h. Cellular ferritin level was measured by a mouse ferritin ELISA assay. Results are normalized to total protein (ng/μg) and presented as mean ± SD (n = 3). “ns” means the difference was not significant. *p < 0.05, **p < 0.01, ***p < 0.001.