Table 3. Biochemical characteristics of DHN3 protein from H. spontaneum and the closely related cereals listed in Materials and Methods section.
| Species | Access No. | Type | No. ofresidues | MW(kDa) | PKCNo | CK2No | pI | GRAVY | AI | II |
|---|---|---|---|---|---|---|---|---|---|---|
| H. spontaneum | Consensus | YSK2 | 155–161* | 16.13 | 9–11 | 4 | 8.24 | −1.051 | 33.07 | 13.26 |
| H. vulgare | AF043089.1 | YSK2 | 155 | 15.86 | 10 | 4 | 8.08 | −1.076 | 32.18 | 12.42 |
| T. aestivum | AAL50791 | YSK2 | 160 | 15.97 | 8 | 4 | 8.06 | −0.946 | 36.69 | 16.06 |
| T. urartu | EMS45466.1 | YSK | 178 | 18.93 | 15 | 5 | 10.22 | −0.946 | 40.11 | 41.12 |
| A. tauschii | EMT24840 | YSK2 | 155 | 15.73 | 8 | 5 | 8.83 | −1.125 | 34.06 | 26.81 |
| B. distachyon | XP003574997 | YSK2 | 183 | 18.46 | 6 | 2 | 9.25 | −1.101 | 27.87 | 18.26 |
| Z.mays | CAM56274.1 | YSK2 | 168 | 17.06 | 8 | 3 | 8.78 | −1.145 | 31.43 | 25.27 |
| O. sativa | NP001067843 | YSK2 | 164 | 16.54 | 4 | 4 | 9.27 | −1.033 | 32.26 | 24.32 |
| S. bicolar | AAA19693 | YSK2 | 154 | 16.28 | 6 | 1 | 9.67 | −1.002 | 43.25 | 25.40 |
MW (Molecular Weight), PKC (Protein Kinase C), CK2 (Casein Kinase 2), pI (Isoelectric Point), GRAVY (Grand Average of Hydropathy), AI (Aliphatic Index), and II (Instability Index) were predicted based on amino acid composition. .* Two values due to the presence of 6-aa indel in Exon 1.