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. 2016 Feb 12;6:20547. doi: 10.1038/srep20547

Figure 2. TGFβ1 induces WISP1 in primary human lung fibroblasts (phLFs).

Figure 2

phLFs were treated with TGFβ1 (2 ng/ml) for 8, 24 and 48 hours and the expression of (A) WISP1 and (B) SERPINE1 were analysed using RT-qPCR. The phLFs were treated with TGFβ1 concentrations ranging from 0.5 to 10 ng/ml and the expression of (C) WISP1 and (D) SERPINE1 were analysed using RT-qPCR at 24 hours. WISP1 was significantly upregulated using 2 ng/ml at 24 hours while SERPINE1 was upregulated at 8 hours and at all further timepoints. (E) phLFs were treated with TGFβ1 (2 ng/ml) for 24 and 48 hours and WISP1 secretion was significantly upregulated as measured by ELISA (n = 3–6; *p < 0.05; **p < 0.01; ***p < 0.001; 1-way ANOVA followed by Neuman-Keuls multiple comparison test; compared to respective control).