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. 2016 Feb 12;6:20547. doi: 10.1038/srep20547

Figure 3. TNFα induces WISP1 in primary human lung fibroblasts (phLFs).

Figure 3

The treatment of phLFs with TNFα (10 ng/ml) for 8, 24 and 48 hours was followed by the analysis of the expression of (A) WISP1 and (B) IL8 using RT-qPCR. (C,D) phLFs were treated with TNFα concentrations from 10 to 100 ng/ml and the expression of WISP1 and IL8 was analysed using RT-qPCR. WISP1 and IL8 were significantly increased after 8 hours of TNFα stimulation with 10 ng/ml (E) WISP1 secretion by phLFs after treatment with TNFα (10 ng/ml) for 24 and 48 hours was significantly increased as measured by ELISA (n = 3–4; *p < 0.05; **p < 0.01; ***p < 0.001; 1-way ANOVA followed by Neuman-Keuls multiple comparison test; compared to respective control).