Table 1. Annotated 5′ untranslated sequences for all annotated transcripts were extracted from the reference Chlamydomonas genome and analyzed for potential uORF content.
uORF Classes Present | |||
---|---|---|---|
Number of Transcript Models | 1 | 2 | 3 |
6438 | — | — | — |
769 | + | — | — |
821 | — | + | — |
6693 | — | — | + |
421 | + | + | — |
1600 | + | — | + |
1514 | — | + | + |
1270 | + | + | + |
Sums | |||
4060 | + | ||
4026 | + | ||
11077 | + |
For schematic of classes see Figure 1. Class 1: ATG in 5UT sequence, in-frame with reference CDS, without intervening stop codon; class 2: ATG in 5UT sequence, out-of-frame with reference CDS, without intervening stop codon; class 3: ATG in 5UT sequence, with stop codon in-frame before the reference CDS. A given transcript can in principle have any number of each class. Sums: total transcripts containing at least one of the indicated class of uORFs.