Table 2. Proteins required for the downregulation of the Ribi gene NSR1 in stress that physically interact with TORC1.
| Name | Description | Loc | [NSR1] | Down TOR |
|---|---|---|---|---|
| VAC8 | Vacuolar membrane protein; CVT pathway | C | 2.9 | Yes |
| GYP5 | GTPase-activating protein for Rab proteins; ER to Golgi transport | C | 1.8 | No |
| DAL82 | Positive regulator of allophanate inducible genes | N | 2.6 | No |
| FMP48 | Protein kinase | C/M | 1.7 | No |
| KDX1 | Protein kinase | M | 1.5 | No |
| NNK1 | Protein kinase | C | 1.8 | No |
| SAP185 | Protein that forms a complex with the Sit4p protein phosphatase | C/M | 1.9 | Yes |
| POP2 | RNase of the DEDD superfamily | C | 1.4 | Yes |
| TIF1 | Translation initiation factor eIF4A | C | 1.6 | ? |
| MRPS17 | Mitochondrial ribosomal protein of the small subunit | C | 1.5 | ? |
| GAS1 | Beta-1,3-glucanosyltransferase | C/M/N | 1.1 | ? |
| HXT2 | High-affinity glucose transporter of the major facilitator superfamily | 1.9 | ? | |
| ICL1 | Isocitrate lyase | C | 2.1 | No |
| SAC6 | Fimbrin, actin-bundling protein | C | 1.8 | No |
| TPO3 | Polyamine transporter of the major facilitator superfamily | C | 1.7 | ? |
| YKU80 | Subunit of the telomeric Ku complex (Yku70p-Yku80p) | N | 1.5 | ? |
| YLR108C | Protein of unknown function | N | 1.8 | Yes |
The third column lists the localization (Loc) of each protein. The fourth column [NSR1] lists the log2 NSR1/PEX6 expression ratio from the screen. The fifth column notes if the gene/protein acts downstream of TORC1 (has log2 > 1 normalized NSR1/PEX6 ratio in rapamycin). A question mark means that the protein/gene was not analyzed in the rapamycin subscreen. C, cytosol; N, nucleus; M, membrane.